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MicroResp图片20210214.jpg


MicroResp是英国James Hutton公司出品,用于土壤、沉积物、水样中碳源测定的专利性装置。
在环境、土壤、水资源保护等领域应用广泛。


MicroResp™ is a unique microplate-based respiration system that enables the user to analyse up to 96 soil, sediment or water samples and test a range of carbon sources and/or replicates in a small compact space.

While some sole C-source tests tend to select for fast-growing bacteria and rely on growth of organisms, MicroResp™ gives more immediate responses to these substrates and reflects activity rather than growth by measuring responses in the first 6 hours. MicroResp™ is currently being used to carry out colorimetric detection or 14C detection measuring CO2 evolved from carbon substrate utilisation in soils. This methodology can be adapted to suit any experimental schedule and microplate reader although calibration is required.


MicroResp Starter Kit (CAT#001)

现货销售

微信请联系

Vicbio微信二维码.jpg


也可通过丁香通商城下单:

https://www.biomart.cn/infosupply/84712339.htm

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说明书请见附件

MicroResp Starter Kit Cat#001 产品明书.pdf

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产品视频

WeChat_20210912230532.mp4


→MicroResp检测原理

http://soils.issas.ac.cn/html/tr/2023/3/tr202207260334.htm#outline_anchor_10


→MicroResp操作视频

https://www.youtube.com/watch?v=IqWOp21My6c


什么是MicroResp™?

MicroResp™是一个独特的基于微孔板的呼吸系统,它使用户能够在一个小且紧凑的空间内分析多达96个土壤、沉积物或水样,

并测试一系列碳源以及进行重复实验。


虽然一些仅针对C-源的测试倾向于选择快速生长的细菌并依赖生物体的生长,但MicroResp™能在前6小时内对这些底物做出更

快速的响应,并通过测量响应来反映活性而非生长。


MicroResp™目前被用于进行比色检测或14C检测,以测量土壤中由碳底物利用产生的CO2。虽然需要校准,但这种方法可以

根据任何实验计划和微孔板读数器进行调整。


MicroResp™主要由三个主要组件组成:

96孔1.2ml深孔板——用于装载碳源和测试材料。

96孔微孔板——包含检测凝胶或在14C检测的情况下,包含用NaOH浸湿的滤纸的96孔等位板。

MicroResp™密封垫——将两块板连接在一起的双向橡胶密封垫。


MicroResp™系统的另外两个必要部分:

夹具——在孵育过程中将三个主要组件固定在一起,确保各孔之间密封良好。

填充装置——帮助用户用固体材料(如土壤)填充深孔板。

虽然MicroResp™是为了在25℃下短时间内(4-6小时)测量CO2的释放而开发的,但它可以根据样品活性和任何温度下的孵育时间进行调整,以模拟自然环境条件。


已证明的应用领域:

土壤健康

毒性测试

农药降解谱

群落水平生理谱(CLPP)

污染诱导的群落耐受性(PICT)

生物修复评价

水生态和毒性


其他潜在应用:

微生物菌株表型分析

动物饲料消化率

种子发芽试验

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MicroResp Starter Kit的主要组件包括:

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